Improved method of analysis for aluminum in brain tissue.

To improve the accuracy and precision of the assay of aluminum in brain tissue, we modified for application to brain samples from rats and humans the wet-digestion method of Trapp et al. (Biol Psychiatry 1978; 13:709-18), established the contribution of contamination, and examined the effect of precipitation of nonoxidizable fatty residues on the analysis. Specifications of the modified assay are a detection limit of 5 ng of aluminum per gram wet weight of brain tissue, a within-day CV of 4.8% (24.3 microgram/L; n = 10), and a day-to-day CV of 5.5% (27.8 micrograms/L; n = 5). Contamination, a systematic error in the analysis of aluminum, was established to be 13 ng (SD = 7.9 ng; n = 8) per tube. The presence of indestructible fatty residues did not affect the accuracy of the method. Application of the method to brain hemispheres of nonexposed rats revealed an aluminum content of 0.041 mg/kg wet weight of tissue (SD = 0.032 mg/kg; n = 8). The aluminum content in human cortex samples, consisting of gray and white matter, ranged from 0.14 to 0.22 mg/kg. Modification of the wet-digestion method resulted in a reliable, simple sample pretreatment before analysis for aluminum in brain tissue. The extent of the aluminum contamination must be controlled by including appropriate blanks.